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DNA Barcoding

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How it works

DNA Barcoding aims to characterise species at the interspecific level, using a short DNA sequence from a standard marker accepted in the genome. It is a molecular species detection tool based on the analysis of standardised genetic sequences. (Herbert et al. 2003). In higher animals, the 5′-region of the mitochondrial cytochrome oxidase (COI) gene I is considered to be a highly efficient genetic “barcode”. In this context, DNA Barcoding is applicable to the detection of species from any properly preserved tissue (fins, muscles, scales, eggs, etc.) and at all life stages (larvae, juveniles and adults). (Becker et al. 2011)

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SPECIES ?

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DNA

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Barcodes

microsatellites

BENEFIT

Interests

  • Applicability to all stages (larvae, juveniles and adults) and to both sexes
  • Fast treatment and low cost
  • Accessible public databases (Genbank BOLD)
  • Re-interpretable and reproducible data
DNA BARCODING

Limits

  • Effectiveness sensitive to sample preservation
  • Amplification of non-target DNA
  • For some taxa, non exhaustive public databases
  • Boundary between intra- and inter

Our publications

Research Articles:

  • Pintilioaie A.-M., et al. (2026). A New Record of the Non-native Species Atypophthalmus (Atypophthalmus) umbratus (Diptera: Tipuloidea) in Romania, with COI-sequenced Specimens. Acta Zoologica Bulgarica, 78(1), 1–7. 
  • Rousset Z. et al. (2025). Spotted! Ophraella communa Le Sage, 1986 (Coleoptera, Chrysomelidae), the ragweed leaf beetle, makes its French debut. BioInvasions Records, 14(4), 833–843. https://doi.org/10.3391/bir.2025.14.4.07
  • Viciriuc I. M. et al. (2025). State of the spread of the exotic parasitoid wasp Leptopilina japonica tracking the route of its invasive host fly Drosophila suzukii in France. Agricultural and Forest Entomology. https://doi.org/10.1111/afe.70025
  • Leblalta et al. (2024). First detection of the opuntia cochineal scale Dactylopius opuntiae (Cockerell, 1896) (Hemiptera: Dactylopiidae) in Misserghin (Oran),  North-West Algeria. 

Conference Posters :

  • IDMABIO: A Platform for the Molecular Characterization of Biological Agents and Pests. IEIC 2025.
  • The utilization of DNA barcoding in the study of parasitoid communities of Cydia pomonella. IEIC 2025.

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