DNA Barcoding
How it works
DNA Barcoding aims to characterise species at the interspecific level, using a short DNA sequence from a standard marker accepted in the genome. It is a molecular species detection tool based on the analysis of standardised genetic sequences. (Herbert et al. 2003). In higher animals, the 5′-region of the mitochondrial cytochrome oxidase (COI) gene I is considered to be a highly efficient genetic “barcode”. In this context, DNA Barcoding is applicable to the detection of species from any properly preserved tissue (fins, muscles, scales, eggs, etc.) and at all life stages (larvae, juveniles and adults). (Becker et al. 2011)
SPECIES ?
DNA
Barcodes
BENEFIT
Interests
- Applicability to all stages (larvae, juveniles and adults) and to both sexes
- Fast treatment and low cost
- Accessible public databases (Genbank BOLD)
- Re-interpretable and reproducible data
DNA BARCODING
Limits
- Effectiveness sensitive to sample preservation
- Amplification of non-target DNA
- For some taxa, non exhaustive public databases
- Boundary between intra- and inter
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