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Microsatellites

short-read-sequencing

How it works

Microsatellites are hypervariable, neutral and codominant markers used for intraspecific population genetics (Avise, 1994). Microsatellites are repeated sequences (sets of 2 or 3 nucleotides for instance) distributed all along the nuclear genome. At each microsatellite locus, the variability in the observed number of repeated sequences determines the number of “alleles”. In diploid organisms, loci can be homozygous (two identical alleles) or heterozygous (two different alleles). The genotyping of one individual allows the identification of the alleles present at each locus.
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Population

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dna
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DNA

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microsatellites
microsatellites

Microsatellites

microsatellites

BENEFIT

Interests

  • Precise characterization of individuals and strains
  • Detection of contamination between strains of the same species
  • Reproducibility (Borém and Caix-eta 2016) and Sylvie W.

Microsatellites

Limits

  • Specific-species development (Larson, Shawn E. et al. 2017) & Sylvie W
  • Presence of “null alleles” (unamplified alleles during genotyping)
  • Risk of Homoplasy (Rousset 1996; Estoup at al. 2002; Bhargava and Fuentes 2010; Haal and Payseur 2011)
  • Possible genetic linkage between some locis (K. A. Selkoe and R. J. Toonen, 2006)
  • Expertise required in population genetics for some advanced applications

Our publications

Research Articles:

  • Pintilioaie A.-M., et al. (2026). A New Record of the Non-native Species Atypophthalmus (Atypophthalmus) umbratus (Diptera: Tipuloidea) in Romania, with COI-sequenced Specimens. Acta Zoologica Bulgarica, 78(1), 1–7. 
  • Rousset Z. et al. (2025). Spotted! Ophraella communa Le Sage, 1986 (Coleoptera, Chrysomelidae), the ragweed leaf beetle, makes its French debut. BioInvasions Records, 14(4), 833–843. https://doi.org/10.3391/bir.2025.14.4.07
  • Viciriuc I. M. et al. (2025). State of the spread of the exotic parasitoid wasp Leptopilina japonica tracking the route of its invasive host fly Drosophila suzukii in France. Agricultural and Forest Entomology. https://doi.org/10.1111/afe.70025
  • Leblalta et al. (2024). First detection of the opuntia cochineal scale Dactylopius opuntiae (Cockerell, 1896) (Hemiptera: Dactylopiidae) in Misserghin (Oran),  North-West Algeria. 

Conference Posters :

  • IDMABIO: A Platform for the Molecular Characterization of Biological Agents and Pests. IEIC 2025.
  • The utilization of DNA barcoding in the study of parasitoid communities of Cydia pomonella. IEIC 2025.

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We’re here to answer all your questions and support you with your projects. Whether you want to learn more about our services, collaborate with us, or simply connect, don’t hesitate to reach out.
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