On the fifth international IEIC 2025 conference (https://ieic2025.sciencesconf.org/), Nicolas Ris and Ionela-Madalina Viciriuc will be presenting two posters that spotlight the IDMABIO initiative. The first poster provides an overview of IDMABIO, outlining the services offered, the target stakeholders, and the operational framework of the platform. The second poster shares the latest progress made by our team, reflecting the ongoing momentum and dedication surrounding the project. These contributions mark a significant step in extending IDMABIO’s visibility within the scientific community.
Poster 1 : IDMABIO: A Platform for the Molecular Characterization of Biological Agents and Pests
Sylvie Warot1, Ionela-madalina Viciriuc1, Gnimavo Bonaventure Kenou1, Thibaut
Malausa1, Jean-Claude Streito1, and Nicolas Ris∗1
1Institut Sophia Agrobiotech – Université Nice Sophia Antipolis (1965 – 2019), Centre National de la
Recherche Scientifique, Institut National de Recherche pour l’Agriculture, l’Alimentation et
l’Environnement, Université Côte d’Azur – France
Abstract :
The biodiversity of most agrosystems remains poorly understood due to taxonomic issues (cryptic species, species complexes, biotypes, etc), rapid changes in communities (invasive exotic species, re-emerging native species, etc) and limited diagnostic capabilities in terms of throughput, time and/or price. This context led to the formalisation in 2024 of the IDMABIO platform (https://idmabio.com/), a service open to the various players in biocontrol, whether public research laboratories, agronomic players (Agricultural Technical Institutes, Chambers of Agriculture, FREDON, etc.) or private biocontrol manufacturers. The services offered include DNA barcoding i.e. the use of a short DNA sequence considered necessary and sufficient to affiliate individuals at the species level. Since the launch of IDMABIO, this approach has been successfully applied to a wide range of taxa, crop pests (Coleoptera, Hemiptera, Lepidoptera, Neuroptera, Thysanoptera etc) or biological control agents (predatory bugs as well as egg, larval or pupal parasitoids).
Keywords: Agrosystem, Biodiversity, Biocontrol, DNA Barcoding
Poster 2 : The utilisation of DNA barcoding in the study of parasitoid communities of Cydia pomonella
Ionela-Madalina Viciriuc∗1, Sylvie Warot1, Cristina Vasilita2, Elodie Vercken1, Perrine
Decoeur1, Inessa Buch1, Mélanie Huguet1, Nicolas Ris1, and Nicolas Borowiec1
1INRAE – Université Côte d’Azur, CNRS, Institut Sophia Agrobiotech – France
2Stuttgart State Museum of Natural History, Stuttgart – Germany
Abstract :
Cydia pomonella (Lepidoptera: Tortricidae), commonly known as the codling moth, is a major pest affecting apples, pears, and walnuts worldwide. It has been present in France for over 250 years and became a significant issue with the expansion of orchards in the 18th century. Various parasitoid species have been identified as potential biological control agents, but only a few have a significant impact on codling moth populations.
In the frame of a classical biological control program using the exotic parasitoid Mastrus ridens (Hymenoptera: Ichneumonidae), field samplings using corrugated cardboards and sentinel larvae were carried out on several crops hosting the codling moth (apple, walnut) between 2019 and 2023, across 66 experimental sites in metropolitan France. At least 700 hymenopteran parasitoids were collected, covering several families such as Ichneumonidae, Braconidae, Perilampidae, and Pteromalidae.
Ichneumonidae was the most represented family (approximately 80% of the individuals). Species identification within this family represents a significant challenge due to complex taxonomy and high levels of morphological homoplasy. While experienced taxonomists can often assign specimens to the correct subfamily, genus, or species, DNA analysis becomes invaluable when morphological identification is not possible, especially for pre-imaginal stages. A cost-effective molecular technique using MinION sequencing is used for precise identification of parasitoid communities and is being implemented within the IDMABIO platform (https://idmabio.com/).
Among the identified species, the most abundant was the primary specialized parasitoid Ascogaster quadridentata (Hymenoptera: Braconidae), which attacks codling moth eggs and emerges from larvae in their penultimate stage. Other species frequently found include Liotryphon caudatus, Pristomerus vulnerator (Ichneumonidae), and Perilampus tristis (Perilampidae), illustrating a diverse and promising community of natural enemies for enhancing biological control strategies against C. pomonella.
Keywords: DNA barcoding, Cydia pomonella, parasitoid communities, pest
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